ViralBioinfAsia2022

This introductory lecture has been pre-recorded for the course and is accessible as a YouTube playlist from: here. It consists of five videos:

• Part 1: https://youtu.be/vDYaKvOFh_k 12:47
• Part 2 https://youtu.be/b7cbuw81iU8 4:22
• Illumina Sequencing by Synthesis https://youtu.be/fCd6B5HRaZ8 5:13
• Part 3 https://youtu.be/68tNkPBFsJk 16:50
• Part 4 https://youtu.be/OSBaDkSwBZ0 7:07

You may also wish to watch these two additional videos about Pacific Biosciences SMRT sequencing:

• https://youtu.be/NHCJ8PtYCFc
• https://youtu.be/v8p4ph2MAvI

The total length of the video playlist is about 48 minutes, so we have about 10 minutes for discussion during the one-hour session.

This lecture covers:

• Introduction to DNA sequencing.
• Sequencing by synthesis.
• Second-generation sequencing: 454 and Illumina.
• Paired-read sequencing.
• FASTQ format for sequence data and quality scores.
• Plotting distributions of quality scores using FastQC.
• Single-molecule sequencing methods: Oxford Nanopore and Pacific Biosciences.

Give the breadth of the subject, this lecture really just scratches the surface. There will be opportunities during the course o delve more deeply into some of these topics and discuss with the course training team.

A PDF of the lecture slides is available here.